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KMID : 1007520140230010269
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Food Science and Biotechnology
2014 Volume.23 No. 1 p.269 ~ p.275
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Anti-cancer Activity of the Ethylacetate Fraction from Orostachys japonicus for Modulation of the Signaling Pathway in HepG2 Human Hepatoma Cells
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Lee Gyeong-Seon
Lee Hyeong-Seon
Kim Seon-Hee
Suk Dong-Hee
Ryu Deok-Seon
Lee Dong-Seok
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Abstract
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The molecular mechanisms of the ethylacetate(EtOAc) fraction from Orostachys japonicus (OJE) (includinggallic acid, kaempferol, and quercetin) for anti-canceractivities in HepG2 cells were investigated. Apoptosis wasdetected using morphological observation of nuclear changesand investigation of phosphatidylserine exposure at thecytoplasmic membrane using FITC-Annexin V/PI staining.Activities of the apoptotic factors bcl-2, bax, cytochrome c,pro-caspase-3, 8, and 9, as well as MAPKs levels, weremeasured using western blotting. Some morphologic featuresof apoptosis were identified using confocal microscopy.OJE caused the expression of apoptotic proteins to change,as evidenced by an increased bax/bcl-2 ratio and increasedexpression of cytochrome c, and decreased expressions ofpro-caspase-3, 8, and 9. After HepG2 cells were exposed toOJE, expressions of p-JNK and p-ERK1/2 increased in adose dependent manner. OJE exhibits anti-cancer activityvia apoptosis induction through a mitochondria dependentsignaling pathway in HepG2 cells.
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KEYWORD
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anti-cancer activity, Orostachys japonicas, apoptotic factors, human hepatoma cells
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